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| Carlos,Lanamar de Almeida; Amaral,Kenas Aguiar da Silva; Vieira,Ivo José Curcino; Mathias,Leda; Braz-Filho,Raimundo; Samarão,Solange Silva; Vieira-da-Motta,Olney. |
| Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rauvolfia grandiflora; Staphylococcus aureus; Enterotoxin; Antimicrobial activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011 |
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| Vieira,Ivo J.C.; Medeiros,Walter L.B.; Monnerat,Cecilia S.; Souza,Jucimar J.; Mathias,Leda; Braz-Filho,Raimundo; Pinto,Angelo C.; Sousa,Priscila M.; Rezende,Claudia M.; Epifanio,Rosângela De A.. |
| The pharmacotherapyfor Alzheimer's disease (AD) includes the use of acetylcholinesterase inhibitors (AChEI). Recent investigations for novel AD therapeutic agents from plants suggested that Tabernaemontana genus is a promising source of novel anticholinesterasic indole alkaloids. In this work two fast screening techniques were combined in order to easily identify novel cholinesterase inhibitors (ChEI). Gas chromatography-mass spectrometry (GC-MS) of the less polar alkaloidic fractions obtained from the acid-base extraction of the stalk of T. laeta revealed thirteen monoindole alkaloids, four of them confirmed by co-injection with previously isolated alkaloids. The others were tentatively identified by mass fragmentation analysis. By gas chromatography with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tabernaemontana; Indole alkaloids; Cholinesterase inhibitor; Thin-layer chromatography assay; Ellman´s method; GC-MS. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000300003 |
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